Examining the Impact of 2-4 D Amine Herbicides on Barbus Luteus Hamri Fish (Heckel)
DOI:
https://doi.org/10.54536/ajec.v4i1.3083Keywords:
2-4 D Amine, Hamri Fish, Herbicide, LC50, HardnessAbstract
The study investigated the herbicide 2-4-D-amine effects on Barbus luteus Hamri fish. The carp were divided into two age categories. The study also measured water properties like water temperature, pH, EC, D.O., and hardness when the fish were exposed to the pesticide.
The study reveals that fish exposed to pesticides show surface symptoms like skin and gill bleeding, neck warping, and scale loss. Compared to a control sample, these fish show irritation or confusion. The acute toxicity (LC50) of the 2-4 D amine was determined for a period of 96 hours after the food was cut off (24 hours) prior to the experiment. The first category has a greater impact on fish than the second, as demonstrated by the statistical analysis results at the probability level (P<0.05), which indicated an inverse link between pesticide toxicity and fish age at usage. The results showed that the higher the pesticide concentration, the higher the mortality rate. The statistical analysis also showed a positive correlation between both categories and the pesticide concentration and the percentage of death (toxicity).
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Abd-Algadir, M., Idris, O., & Elkhier, M. (2011). Effect of Pendimethalin herbicide on fish (Tilapia nilotica) skeletal muscles, gills and its influence on human. World Journal of Life Sciences and Medical Research, 3(1), 5–10.
Akhtar, M. (1986). The disposition of 14C agrochemical residues in plant and animal tissues when consumed by animals. In T. Greenhalgh & T. R. Roberts (Eds.), Pesticide science and biotechnology: Proceedings of the 6th international congress of pesticide chemistry, Ottawa, Canada (pp. 10–15).
Annune, P., Hbele, S., & Oladimeji, A. (1994). Acute toxicity of cadmium to juveniles of Clarias gariepinus (Tuegls) and Oreochromis niloticus (Trewavas). Journal of Environmental Science and Health, A29, 1357–1365.
Antychowicz, J., Szymbor, E., & Roszkowski, J. (1979). Investigations upon the effects of some pesticides on carp (Cyprinus carpio). Bulletin of the Veterinary Institute in Pulawy, 23(3/4), 124–130.
APHA. (2005). Standard methods for the examination of water and wastewater (21st ed.). Author.
Banaee, M. (2012). Adverse effect of insecticides on various aspects of fish’s biology and physiology. In Insecticides - Basic and other applications (pp. 102–126). IntechOpen.
Dewey, S. (1986). Effects of the herbicide atrazine on aquatic insect community structure and emergence. Ecology, 67, 148–162.
Dhurgham, A., Firas, M., & Abbas, T. (2019). Effects of contaminated water with glyphosate herbicides on the external and behavioral characteristics of common carp, Cyprinus carpio Linnaeus. Biochemical and Cellular Archives, 19(1), 1475–1480.
Edward, A., & Thompson, B. (1973). Persistent pesticides in the environment (2nd ed.). Chemical Rubber Co. Press.
Ervnest, H. (2004). A textbook of modern toxicology (3rd ed.). John Wiley & Sons.
Finney, D. (1952). Probit analysis: A statistical treatment of the sigmoid response curve. Cambridge University Press: Cambridge, UK.
Finney, D. T. (1971). Probit analysis. Cambridge University Press.
Fulton, M., & Key, P. (2001). Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environmental Toxicology and Chemistry, 20, 37-45.
Gomez, L., Masot, J., Martínez, S., Durán, E., Soler, F., & Roncero, V. (1998). Acute 2,4-D poisoning in tench (Tinca tinca L.): Lesions in the haematopoietic portion of the kidney. Archives of Environmental Contamination and Toxicology, 35, 479-483.
Guiho, M. (1995). Les réseaux de surveillance résultat, évolution. In E. Bretagne (Ed.), Qualité des eaux et produits phytosanitaires: Du diagnostic à l’action (p. 19). Rennes, France.
Halter, M. (1980). 2,4-D in the aquatic environment. In R. Shearer & M. Halter (Eds.), Literature reviews of four selected herbicides: 2,4-D, dichlobenil, diquat & endothall (Section II).
Hameed, A., & Ahmed, J. (2016). Acute and chronic effects of water soluble fraction (WSF) of diesel fuel on common carp (Cyprinus carpio L. 1758). Journal of International Environmental Application & Science, 11(4), 331-345.
Herrera, A., & Catap, E. S. (2001). Toxicity of the pesticide Azinphosethyl on various organs of juvenile Cyprinus carpio. Asian Life Sciences, 10(2), 83-97.
Hussein, S. Y., El Nasser, M., & Ahmed, S. (1996). Comparative studies on the effects of herbicide atrazine on freshwater fish Oreochromis niloticus and Chrysichthyes auratus at Assiut, Egypt. Bulletin of Environmental Contamination and Toxicology, 57, 503-510.
Jiraungkoorskul, W., Upatham, E., & Kruatrachue, M. (2002). Histopathological effects of Roundup, a glyphosate herbicide, on Nile tilapia (Oreochromis niloticus). ScienceAsia, 28, 121-127.
Lind, G. T. (1979). Handbook of common methods in limnology (2nd ed.). London.
Mackereth, F., Heron, J., & Talling, J. T. (1978). Water analysis: Some revised methods for limnologists. Scientific Publications, Freshwater Biological Association (England), 36, 1-120.
Marie, M., Mohamed, H., & El-Badawy, A. (1998). Physiological and biochemical responses of the common carp, Cyprinus carpio, to an organophosphorus insecticide “Profenofos”. Egyptian Journal of Zoology, 31, 297-302.
Mason, C. (1991). Biology of freshwater pollution (2nd ed.). Longman Scientific and Technical: U.K.
Oemichen, U., & Haberer, K. (1986). Stickstoffherbizide im Rhein. Vom Wasser, 66, 225-241.
Oliveira, A., Belger, L., Pelletier, E., & Rouleau, C. (2002). Histopathological evidence of inorganic mercury and methyl mercury toxicity in the arctic charr (Salvelinus alpinus). Environmental Research, 90, 217-225.
Osfor, M., Abdel Wahab, A., & Dessouki, S. (1998). Occurrence of pesticides in fish tissues, water, and soil sediment from Manzala Lake and River Nile. Nahrung, 42(1), 39-41.
Pant, J., Tewari, H., & Gill, T. (1987). Effects of aldicarb on the blood and tissues of a freshwater fish. Bulletin of Environmental Contamination and Toxicology, 38, 36-41.
Phillips, T., Wu, J., Summerfelt, R., & Atchison, G. (2002). Acute toxicity and cholinesterase inhibition in larval and early juvenile walleye exposed to chlorpyrifos. Environmental Toxicology and Chemistry, 21(7), 1469-1474.
Que Hee, S., & Sutherland, R. (1981). The phenoxyalkanoic herbicides, Volume I: Chemistry, analysis, and environmental pollution. CRC Press, Inc.: Boca Raton, FL.
Sastry, K., Siddiqui, A., & Samuel, M. (1988). Acute and chronic toxic effects of the carbamate pesticide sevin on some hematological, biochemical, and enzymatic parameters in the freshwater teleost fish Channa punctatus. Acta Hydrochimica et Hydrobiologica, 16, 625-631.
Segnini, I., Medina, J., & Marcano, S. (2005). Effects of herbicide on the kidneys of two Venezuelan cultured fish: Caquetaia kraussii and Colossoma macropomum (Pisces: Cichlidae and Characidae). International Journal of Tropical Biology, 53(1), 55-59.
Shafer, H., Hettler, H., & Fritsche, U. (1994). Biotests using unicellular algae and ciliates for predicting long-term effects of toxicants. Ecotoxicology and Environmental Safety, 27, 64-81.
Shaffi, S. (1979). The acute toxicity of heptachlor to freshwater fishes. Toxicology Letters, 4, 31.
Shaikila, B., Thangavel, P., & Ramaswamy, M. (1993). Adaptive trends in tissue acid and alkaline phosphatases of Sarotherodon mossambicus (Peters) under Sevin toxicity. Indian Journal of Environmental Health, 35(1), 36-39.
U.S. EPA. (2005). Reregistration eligibility decision for 2,4-D. Office of Prevention, Pesticides and Toxic Substances, U.S. Environmental Protection Agency. Retrieved from http://www.epa.gov/oppsrrd1/REDs/24d_red.pdf
Wang, Y., Yen, J., & Hsieh, Y. (1994). Dissipation of 2,4-D, glyphosate, and paraquat in river water. Water, Air, & Soil Pollution, 72, 1-7.
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Copyright (c) 2025 Dhurgham A.A. AL-Sultany, Ladha Mohammed Obayes, Laith Taha Mohammed, Tabarek Najah Hadi Al-Gburi
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